【摘要】 目的 探討抗氧化應(yīng)激是否參與參附注射液預(yù)處理誘導(dǎo)的腎臟保護作用。 方法 健康成年雄性SD大鼠21只隨機分為假手術(shù)對照組（Sham組）、腎臟缺血再灌注組（I/R組）和參附注射液組（SF組）；SF組給予參附注射液10 mL/kg腹腔注射，每日1次，連續(xù)給藥7d。麻醉下行右腎切除后，用無損傷動脈夾鉗夾左側(cè)腎蒂60min，再灌注24 h，制備腎缺血再灌注損傷動物模型。比較各組SD大鼠再灌注24 h腎臟組織中超氧化物歧化酶（superonidedismutase，SOD）水平、過氧化氫酶（catalese，CAT）和丙二醛（malonicalaldehyed，MDA）含量。 結(jié)果 與Sham組相比，I/R和SF組腎臟組織SOD和CAT顯著降低，而MDA明顯升高（P lt;0.05）；與I/R組比，參附注射液能明顯增加SOD和CAT水平（P lt;0.05），降低MDA含量（P lt;0.05）。 結(jié)論 參附注射液預(yù)處理可增強缺血再灌注損傷腎臟組織抗氧化應(yīng)激，其表現(xiàn)為增強SOD和CAT的活力，減少MDA的生成。
【Abstract】 Objective To explore the protective effect of Shenfu injection combined with antioxidant system on rats’ kidney after ischemia-reperfusion injury. Methods Twenty-one male Sprague Dawley (SD) rats were randomly divided into 3 groups: sham operation group (Sham group), ischemia-reperfusion group (IR group), and shenfu injection treated group (SF group). The rats were anesthetized with valebarbitone. Bilateral kidneys were exposed through midline incision. The right kidney underwent the nephrectomy and left renal pedicels were occluded for 60 minutes with a traumatic mini-clamp and then unclamped for 24 hours. Animals in SF group received Shenfu injection (10 mL/kg) through intraperitoneal injection every day for 7 days. About 24 hours after reperfusion, superoxide dismutase (SOD), CAT and malonical aldehyde (MDA) were measured. Results The levels of MDA were lower in SF group than those in IR group (P lt;0.05). The level of SOD and CAT in SF group increased more significantly than which did in IR group (P lt;0.05). Conclusion Our finding suggests that antioxidant system in SF group works more efficiently than IR group to overcome oxidative stress in renal ischemia-reperfusion injury.
引用本文： 李毅,王穎,李玨,張廷模. 抗氧化應(yīng)激參與參附注射液預(yù)處理誘導(dǎo)的腎臟保護作用. 華西醫(yī)學(xué), 2011, 26(1): 31-33. doi: 復(fù)制
| 1. | 朱玲, 周黎明, 王正榮. 參附注射液的作用及作用機制的研究進展[J]. 四川生理科學(xué)雜志, 2004, 26 (2): 77-82. |
| 2. | Zheng CD, Min S. Cardioprotection of shenfu injection against myocardial ischemia/reperfusion injury in open heart surgery[J]. Chin J Intergr Med, 2008, 14(1): 10-16. |
| 3. | Wang YL, Wang CY, Zhang BJ， et al. Shenfu injection suppresses apoptosis by regul-ation of Bcl-2 and caspase-3 during hypoxia/reoxygenation in neonatal rat cardiomyo-cytes in vitro[J]. Mol Biol Rep, 2009, 36(2): 365-370. |
| 4. | 王東，朱繼業(yè)，冷希圣. 參附注射液抗肝臟缺血/再灌注損傷的實驗研究[J]. 中國中西醫(yī)結(jié)合雜志，2006（S1）：61-63. |
| 5. | 王軍，楊麗娟，周長美，等. 參附注射液對新生大鼠缺氧缺血性腦損傷干預(yù)作用的實驗研究[J]. 中華醫(yī)學(xué)雜志, 2006, 86(42): 2994-2997. |
| 6. | Dogan Z, Yuzbasioglu MF, Kurutas EB, et al. Thiopental improves renal ischemia-reperfusion injury in rats[J]. Ren Fail, 2010, 32(3): 391-395. |
| 7. | Yun Y, Duan WG, Chen P, et al. Ischemic postconditioning modified renal oxidative stress and lipid peroxidation caused by ischemic reperfusion injury in rats[J]. Transplant Proc, 2009, 41(9): 3597-3602. |
- 1. 朱玲, 周黎明, 王正榮. 參附注射液的作用及作用機制的研究進展[J]. 四川生理科學(xué)雜志, 2004, 26 (2): 77-82.
- 2. Zheng CD, Min S. Cardioprotection of shenfu injection against myocardial ischemia/reperfusion injury in open heart surgery[J]. Chin J Intergr Med, 2008, 14(1): 10-16.
- 3. Wang YL, Wang CY, Zhang BJ， et al. Shenfu injection suppresses apoptosis by regul-ation of Bcl-2 and caspase-3 during hypoxia/reoxygenation in neonatal rat cardiomyo-cytes in vitro[J]. Mol Biol Rep, 2009, 36(2): 365-370.
- 4. 王東，朱繼業(yè)，冷希圣. 參附注射液抗肝臟缺血/再灌注損傷的實驗研究[J]. 中國中西醫(yī)結(jié)合雜志，2006（S1）：61-63.
- 5. 王軍，楊麗娟，周長美，等. 參附注射液對新生大鼠缺氧缺血性腦損傷干預(yù)作用的實驗研究[J]. 中華醫(yī)學(xué)雜志, 2006, 86(42): 2994-2997.
- 6. Dogan Z, Yuzbasioglu MF, Kurutas EB, et al. Thiopental improves renal ischemia-reperfusion injury in rats[J]. Ren Fail, 2010, 32(3): 391-395.
- 7. Yun Y, Duan WG, Chen P, et al. Ischemic postconditioning modified renal oxidative stress and lipid peroxidation caused by ischemic reperfusion injury in rats[J]. Transplant Proc, 2009, 41(9): 3597-3602.