• 四川大學(xué)華西醫(yī)院感染性疾病中心，生物治療國(guó)家重點(diǎn)實(shí)驗(yàn)室感染性疾病研究室，四川成都 610041;

目的：建立能表達(dá)乙肝病毒（hepatitis B virus,HBV）preS2S抗原蛋白的荷瘤小鼠模型，為研究HBV核酸疫苗的體內(nèi)CTL應(yīng)答及免疫治療作用提供簡(jiǎn)便易行的研究模型。 方法：以免疫印跡法驗(yàn)證SP2/0-S2S細(xì)胞中有HBV preS2S抗原的穩(wěn)定表達(dá)，將SP2/0-S2S細(xì)胞種植到BALB/c小鼠脅部皮下（荷瘤），觀察能否生長(zhǎng)成瘤，以及成瘤的時(shí)間、腫瘤大小和荷瘤后小鼠的生存時(shí)間；以免疫組織化學(xué)法檢測(cè)小鼠腫瘤組織HBV preS2S抗原的表達(dá)。以不表達(dá)preS2S抗原蛋白的SP2/0-CMV細(xì)胞荷瘤小鼠作為陰性對(duì)照。結(jié)果：荷瘤后3天～1周，SP2/0-S2S細(xì)胞可在小鼠皮下形成實(shí)體腫瘤，成瘤率為100%，腫瘤細(xì)胞中有preS2S抗原表達(dá)，荷瘤后小鼠的平均生存時(shí)間為16±1天；與不表達(dá)preS2S抗原蛋白的SP2/0-CMV細(xì)胞荷瘤小鼠相比，成瘤率、成瘤時(shí)間、腫瘤大小及生存時(shí)間差異。結(jié)論：建立了能表達(dá)HBV preS2S抗原蛋白的荷瘤小鼠模型，可用于HBV核酸疫苗的體內(nèi)CTL應(yīng)答及免疫治療作用的實(shí)驗(yàn)研究。同時(shí)也建立了不表達(dá)preS2S抗原蛋白的荷瘤小鼠模型，可用作陰性對(duì)照。

引用本文： 何芳,唐紅,劉麗,劉聰,趙連三. 表達(dá)乙肝病毒preS2S蛋白的荷瘤小鼠模型的建立. 華西醫(yī)學(xué), 2008, 23(1): 96-98. doi: 復(fù)制

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