• 四川大學華西醫(yī)院 1呼吸內(nèi)科;2實驗醫(yī)學科，四川成都 610041;

研究白介素6(IL-6)在過氧化氫誘導的肺泡上皮細胞凋亡中的作用及其機制。方法：以人Ⅱ型肺泡上皮細胞A549細胞株為實驗對象，MTT法檢測IL-6對A549細胞增殖活性的影響，TUNEL檢測法檢測IL-6對A549細胞凋亡率的影響，Western blot觀察caspase-3和caspase-9蛋白量的變化。結(jié)果：IL-6能夠增加A549細胞的增殖活性，減少其凋亡。與模型組相比，IL-6干預組的caspase-3和caspase-9的蛋白表達量明顯降低,有統(tǒng)計學意義。結(jié)論：IL-6能夠抑制過氧化氫誘導的肺泡上皮細胞凋亡，并且與caspase-3和caspase-9蛋白的表達量下降有關(guān)。提示IL-6對肺泡上皮細胞的保護作用可能是通過抑制細胞凋亡相關(guān)蛋白來實現(xiàn)的。

引用本文： 楊冬梅,丁宇,應斌武,蔣飛,美朗曲措,范紅. 白介素6對過氧化氫誘導的肺泡上皮細胞凋亡的影響. 華西醫(yī)學, 2008, 23(1): 98-100. doi: 復制

1. Simpson RJ,Hammacher A,Smith DK,et al.Interleukin-6:structure-function relationships[Ｊ].Protein Sci，1997,6（５）:925-955..
2. Waxman AB,Mahboubi K,Knickelbein RG,et al.Interleukin-11 and interleukin-6 protect cultured human endothelial cells from H2O-2-induced celldeath[J].Am J Respir Cell Mol Biol,2003,29(4):513-522..
3. 汪家政，范明.蛋白質(zhì)技術(shù)手冊[Ｊ]．第1版.北京：科學出版社，2001：51-54．.
4. Jin XL,Zimmers TA,Perez EA,et al.Paradoxical effects of short-and long-term interleukin-6 exposure on liver injury and repair[J].Hepatology,2006,43(3):474-484..
5. Yamashita T,Sawamoto S，Suzuki S,et al.Blockade of interleukin-6 signaling aggravates ischemic cerebral damage in mice:possible involvement of Stat3 activation in the protection of neurons[J].J Neurochem，2005,94(2):459-468.
6. Hengartner MO.The biochemistry of apoptosis[Ｊ].Nature,2000,407:770-776..
  1. 1. Simpson RJ,Hammacher A,Smith DK,et al.Interleukin-6:structure-function relationships[Ｊ].Protein Sci，1997,6（５）:925-955..
  2. 2. Waxman AB,Mahboubi K,Knickelbein RG,et al.Interleukin-11 and interleukin-6 protect cultured human endothelial cells from H2O-2-induced celldeath[J].Am J Respir Cell Mol Biol,2003,29(4):513-522..
  3. 3. 汪家政，范明.蛋白質(zhì)技術(shù)手冊[Ｊ]．第1版.北京：科學出版社，2001：51-54．.
  4. 4. Jin XL,Zimmers TA,Perez EA,et al.Paradoxical effects of short-and long-term interleukin-6 exposure on liver injury and repair[J].Hepatology,2006,43(3):474-484..
  5. 5. Yamashita T,Sawamoto S，Suzuki S,et al.Blockade of interleukin-6 signaling aggravates ischemic cerebral damage in mice:possible involvement of Stat3 activation in the protection of neurons[J].J Neurochem，2005,94(2):459-468.
  6. 6. Hengartner MO.The biochemistry of apoptosis[Ｊ].Nature,2000,407:770-776..