• 1.成都軍區(qū)總醫(yī)院全軍普外中心（成都610083）;;
  • 2. 成都軍區(qū)總醫(yī)院臨床實(shí)驗(yàn)科;

目的觀察急性胰腺炎發(fā)生時(shí)肝組織中核因子κB（NFκB）對TNFα mRNA表達(dá)的調(diào)節(jié)及其在肝損傷中的作用。方法Wistar大鼠72只，隨機(jī)均分為急性胰腺炎組（AP組）、急性胰腺炎吡咯基二硫氨基甲酸酯（PDTC）處理組（APP組）以及對照組（SO組）。分別在術(shù)后3 h、6 h、12 h及24 h檢測肝組織NFκB活性、TNFα mRNA的表達(dá)以及血漿ALT水平。結(jié)果AP組及APP組的NFκB活性、TNFα mRNA表達(dá)及血漿ALT水平分別在術(shù)后3～6 h及3～24 h顯著高于SO組。在應(yīng)用了NFκB抑制劑的APP組，NFκB活性、TNFα mRNA表達(dá)以及血漿ALT水平均顯著低于AP組。結(jié)論急性胰腺炎發(fā)生時(shí)，肝臟中活化的NFκB促進(jìn)了TNFα mRNA的表達(dá)，并參與了肝損傷的發(fā)生。

引用本文： 石力,田伏洲,李旭,黃大熔,趙碧,顧大勇. 急性胰腺炎大鼠肝臟NFκB對TNFα表達(dá)的調(diào)控及其在肝損傷中的作用. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(3): 228-230. doi: 復(fù)制

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  1. 1. Miyahara S, Isaji S. Liver injury in acute pancreatitis and mitigation by continuous arterial infusion of an antibiotic via the superior mesenteric artery [Ｊ]. Pancreas, 2001; 23(2)∶204.
  2. 2. 石力， 黃大熔， 田伏洲， 等. 急性胰腺炎大鼠肝臟核因子κB活化與肝損傷的關(guān)系 [Ｊ]. 世界華人消化雜志， 2002； 10（5）∶554.
  3. 3. Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [Ｊ]. J Surg Res, 2000; 90(2)∶95.
  4. 4. Deryckere F, Gannon F. A onehour minipreparation technique for extraction of DNAbinding proteins from animal tissues [Ｊ]. Biotechniques, 1994; 16(3)∶405.
  5. 5. Chomczynski P, Sacchi N. Singlestep method of RNA isolation by acid guanidinium thiocyanatephenolchloroform extraction [Ｊ]. Anal Biochem, 1987; 162(1)∶156.
  6. 6. Pitchumoni CS, Agarwal N, Jain NK. Systemic complications of acute pancreatitis [Ｊ]. Am J Gastroenterol, 1988; 83(6)∶597.
  7. 7. Valen G, Yan ZQ, Hansson GK. Nuclear factor kappaB and the heart [Ｊ]. J Am Coll Cardiol, 2001; 38(2)∶307.
  8. 8. Wright G, Singh IS, Hasday JD, et al. Endotoxin stressresponse in cardiomyocytes: NFkappa B activation and tumor necrosis factoralpha expression [Ｊ]. Am J Physiol Heart Circ Physiol, 2002; 282(3)∶H872.
  9. 9. Moine P, McIntyre R, Schwartz MD, et al. NFkappa B regulatory mechanisms in alveolar macrophages from patients with acute respiratory distress syndrome [Ｊ]. Shock, 2000; 13(2)∶85.
  10. 10. Udalova IA, Knight JC, Vidal V, et al. Complex NFkappa B interactions at the distal tumor necrosis factor promoter region in human monocytes [Ｊ]. J Biol Chem, 1998; 273(33)∶21178.
  11. 11. Essani NA, Fisher MA, Jaeschke H. Inhibition of NFkappa B activation by dimethyl sulfoxide correlates with suppression of TNFalpha formation, reduced ICAM1 gene transcription, and protection against endotoxininduced liver injury [Ｊ]. Shock, 1997; 7(2)∶90.