目的探討生長抑素對人胃癌細胞的抑制作用及其作用機理。方法以生長抑素類似物奧曲肽作用于人胃癌細胞株SGC7901細胞,以人成纖維細胞株HF作為正常細胞對照,以5FU作為陽性藥物對照,通過MTT實驗、熒光顯微鏡觀察及流式細胞術分析,得出結論。結果一定濃度范圍內的奧曲肽對SGC7901細胞產(chǎn)生抑制作用,其抑制作用具有量效關系和飽和性。奧曲肽對SGC7901細胞的抑制程度接近于5FU對他的抑制,而對HF細胞無影響。奧曲肽可誘導SGC7901細胞凋亡。結論奧曲肽在體外對SGC7901細胞具有抑制作用,誘導腫瘤細胞凋亡可能是其作用機理之一。
引用本文: 呂文才,耿小平,孟翔,凌譚,文翔. 奧曲肽對人胃癌細胞的凋亡誘導作用. 中國普外基礎與臨床雜志, 2004, 11(3): 238-241. doi: 復制
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- 2. Tomassetti P, Migliori M, Gullo L. Slowrelease lanreotide treatment in endocrine gastrointestinal tumors [J]. Am J Gastroenterol, 1998; 93(9)∶1468.
- 3. Eriksson B, Renstrup J, Imam H, et al. Highdose treatment with lanreotide of patients with advanced neuroendocrine gastrointestinal tumors: clinical and biological effects [J]. Ann Oncol, 1997; 8(10)∶1041.
- 4. Madeira I, Ruszniewski P. Digestive system carcinoid tumors: treatment [J]. Rev Med Interne, 1999; 20(5)∶421.
- 5. 鄭永唐,賁昆龍. 測定細胞存活和增殖的MTT方法的建立 [J]. 免疫學雜志, 1992; 8(4)∶266.
- 6. Arnold R, Trautmann ME, Creutzfeldt W, et al. Somatostatin analogue octreotide and inhibition of tumour growth in metastatic endocrine gastroenteropancreatic tumours [J]. Gut, 1996; 38(3)∶430.
- 7. Degen L, Beglinger C. The role of octreotide in the treatment of gastroenteropancreatic endocrine tumors [J]. Digestion, 1999; 60(Suppl 2)∶9.
- 8. 鄂征主編. 組織培養(yǎng)和分子細胞學技術 [M]. 第1版. 北京: 北京出版社, 1995∶203~205.
- 9. Di Leo A, Bajetta E, Ferrari L, et al. A dosefinding study of lanreotide (a somatostatin analog) in patients with colorectal carcinoma [J]. Cancer, 1996; 78(1)∶35.
- 10. Janson ET, Stridsberg M, Gobl A, et al. Determination of somatostatin receptor subtype 2 in carcinoid tumors by immunohistochemical investigation with somatostatin receptor subtype 2 antibodies [J]. Cancer Res, 1998; 58(11)∶2375.
- 11. Qin Y, Ertl T, Groot K, et al. Somatostatin analog RC160 inhibits growth of CFPAC1 human pancreatic cancer cells in vitro and intracellular production of cyclic adenosine monophosphate [J]. Int J Cancer, 1995; 60(5)∶694.
- 12. Reisine T. Somatostatin receptors [J]. Am J Physiol, 1995; 269(6 Pt 1)∶G813.
- 13. 吳蘇冬, 劉長利, 王慧川, 等. 參臼膠囊誘導肝癌SMMC7721細胞凋亡 [J]. 世界華人消化雜志, 2003; 11(7)∶908.