目的 探討梗阻性黃疸(簡稱梗黃)大鼠心肌組織中TNF-α及超氧化物歧化酶(SOD) 基因mRNA的表達(dá)。
方法 RT-PCR法擴增梗黃大鼠心肌組織中cDNA,電泳掃描后半定量分析TNF-α和SOD基因mRNA表達(dá)。
結(jié)果 梗黃大鼠心肌組織中TNF-α基因mRNA表達(dá)增強,SOD基因mRNA表達(dá)下降; 血清中TNF-α水平升高,心肌組織中SOD合成減少。
結(jié)論 梗黃心肌的損害與TNF-α基因mRNA表達(dá)增強、SOD基因mRNA表達(dá)降低有關(guān)。
引用本文: 鞏鵬,王忠裕,趙作偉,王洪江,關(guān)鳳林. 梗阻性黃疸大鼠心肌TNF-α及SOD基因mRNA的表達(dá). 中國普外基礎(chǔ)與臨床雜志, 2004, 11(6): 502-504. doi: 復(fù)制
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- 2. Kennedy JA, Lewis H, Clements WD, et al. Kupffer cell blockade, tumour necrosis factor secretion and survival following endotoxin challenge in experimental biliary obstruction [J]. Br J Surg, 1999; 86(11)∶1410.
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