• 1.四川大學(xué)華西醫(yī)院普外科(成都610041);;
  • 2.廣西醫(yī)科大學(xué)附一院普外科(南寧530021);;
  • 3.日本產(chǎn)業(yè)醫(yī)科大學(xué)生態(tài)科學(xué)研究所腫瘤部(Kitakyushu 807-8555,Japan);;
  • 4.廣西醫(yī)科大學(xué)附一院檢驗科;;
  • 5.廣西扶綏縣腫瘤防治研究所(扶綏532100);

的探討一套高效液相色譜電化學(xué)系統(tǒng)檢測尿8羥基脫氧鳥苷(8OHdG)水平的方法學(xué)。方法采用ESA 5600 Coularray系統(tǒng)檢測廣西扶綏縣肝癌戶人群21例及南寧市對照人群63例尿8OHdG水平。結(jié)果8OHdG峰與雜質(zhì)峰分離良好; 8OHdG濃度在0.01~15 ng/ml間呈良好線性; 保留時間、標(biāo)樣和內(nèi)對照的重復(fù)性試驗的批內(nèi)差異(CV)均小于8%; 標(biāo)樣(F=0.002, P=1.000)和內(nèi)對照(F=2.965, P=0.082)的重復(fù)性試驗的批間差異經(jīng)方差分析均無顯著性意義。采用上述方法測得84例總的尿8OHdG水平為(5.26±0.41)ng/mg肌酐,高于同類研究的檢測值〔(4.62±0.091)ng/mg肌酐, n=32〕,但兩地人群間尿8OHdG水平差異無顯著性意義。結(jié)論這是對肝癌高發(fā)區(qū)高危人群氧化性DNA損傷的首次報告。通過謹(jǐn)慎設(shè)計試驗和優(yōu)化試驗參數(shù),該系統(tǒng)對8OHdG峰的分離優(yōu)于以往報告,適用于尿8OHdG的高通量定量分析,可應(yīng)用于人群氧化性DNA損傷的研究。

引用本文: 彭濤,嚴(yán)律南,劉志明,葛西宏,彭民浩,黎樂群. 高效液相色譜電化學(xué)法檢測尿液8羥基脫氧鳥苷水平. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(3): 281-283. doi: 復(fù)制

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  1. 1. Halliwell B. Free radical, antioxidants, and human disease: curiosity, cause, or consequence? [J]. Lancet, 1994; 344(13)∶721.
  2. 2. Grollman AP, Moriya M. Mutagenesis by 8oxoguanine: an enemy within [J]. Trends Genet, 1993; 9(7)∶246.
  3. 3. Kasai H. Analysis of a form of oxidative DNA damage, 8hydroxy2’deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis [J]. Mutat Res, 1997; 387(3)∶147.
  4. 4. Kasai H, IwamotoTanaka N, Miyamoto T, et al. Life style and urinary 8hydroxydeoxyguanosine, a marker of oxidative dna damage: effects of exercise, working conditions, meat intake, body mass index, and smoking [J]. Jpn J Cancer Res, 2001; 92(1)∶9.
  5. 5. Bogdanov MB, Beal MF, McCabe DR, et al. A carbon columnbased liquid chromatography electrochemical approach to routine 8hydroxy2’deoxyguanosine measurements in urine and other biologic matrices: a oneyear evaluation of methods [J]. Free Radic Biol Med, 1999; 27(5-6)∶647.
  6. 6. Tagesson C, Chabiuk D, Axelson O,et al. Increased urinary excretion of the oxidative DNA adduct, 8hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber, and azodye industies [J]. Pol J Occup Med Environ Health, 1993; 6(4)∶357.
  7. 7. Loft S, Vistisen K, Ewertz M, et al. Oxidative DNA damage estimated by 8hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index [J]. Carcinogenesis, 1992; 13(12)∶2241.
  8. 8. Acworth IN, McCabe DR, Maher TJ. The analysis of free radical, their reaction products, and antioxidants. In: Baskin SI, Salem H. Eds. Oxidants, antioxidants, and free radicals [M]. Washington: Taylor & Francis, 1997∶23-77.