• 1.四川大學華西醫(yī)院普外科 (成都610041);;
  • 2.四川大學華西基礎醫(yī)學與法醫(yī)學院生化教研室;;
  • 3.四川大學華西基礎醫(yī)學與法醫(yī)學院檢測中心;;
  • 4.四川大學華西公共衛(wèi)生學院病理科;

目的 探討肝細胞膜鈉泵(Na+K+ATPase)活性變化在膽紅素鈣結石成石過程中的作用。方法采用膽紅素鈣結石兔模型進行研究，對照組28只，膽道不全梗阻組（BO組）36只，膽道不全梗阻加感染組（BOI組）39只，各組再按術后處死時間3 d、7 d、14 d及20 d時相分組。動態(tài)測定各組各時相肝細胞膜鈉泵活性、肝細胞鈣及膽汁中膽汁酸含量。 結果BO組及BOI組肝細胞膜鈉泵活性及膽汁中膽汁酸含量進行性下降，肝細胞鈣含量呈進行性增多，與對照組相比差異有顯著性意義（P＜0.01），但BOI組上述變化較BO組明顯（P＜0.05）。結論膽紅素鈣結石成石過程中存在肝細胞膜鈉泵活性的進行性下降，鈉泵活性下降引起肝細胞鈣超負荷及膽汁中膽汁酸含量下降，從而促進成石。細菌感染加重上述變化，使結石形成增多。

引用本文： 莊文,肖路加,左鳳瓊,東云華,吳忠舜,吳兆鋒. 肝細胞膜鈉泵活性變化在膽紅素鈣結石成石過程中的作用. 中國普外基礎與臨床雜志, 2002, 9(2): 73-75. doi: 復制

版權信息： ?四川大學華西醫(yī)院華西期刊社《中國普外基礎與臨床雜志》版權所有，未經(jīng)授權不得轉載、改編

1. 周孝思. 我國膽色素結石成因的研究狀況 [J]. 普外臨床，1994; 9(4)∶193.
2. 曾勇，肖路加，張尚福. 兔膽紅素鈣結石成石過程中膽囊免疫球蛋白分泌細胞含量的變化 [J]. 普外基礎與臨床雜志，1997; 4(2)∶75.
3. Meeda T, Bismuth H, Sherlock DJ, et al. A simple and rapid method for the preparation of plasma membranes [J]. Biochim Biophys Acta, 1983; 731(5)∶115.
4. Jame L, Merion RM, Burtch GD, et al. Properties of Na+K+activated ATPase in rat liver plasma membranes enriched with bile canaliculi [J]. Biochim Biophys Acta, 1975; 401(3)∶59.
5. Schanne FA, Muller C, Endlich K, et al. Liver plasma membrane calcium transport [J]. J Biol Chem, 1986; 261(21)∶9886.
6. Michael H, Garrel T, Decurtins M, et al. Mechanisms and regulations of bile secretion [J]. Hepatology, 1991; 14(3)∶551.
7. Frimmer M, Grant B, Zobeck TS, et al. The transport of bile acids in liver cells [J]. Biochim Biophys Acta, 1988; 947(8)∶75.
8. Chung TY. Calcium and ischemic injury [J]. New Engl J Med, 1986;314(26)∶1670.
9. Victor A, Inan M, Sayek I, et al. Conditions that results in the mobilization and influx of Ca2+ into rat hepatocytes induce the rapid loss of HMGCoA reductase activity that is not reversed by phosphatase treatment [J]. Biochem J, 1990; 269(9)∶373.
  1. 1. 周孝思. 我國膽色素結石成因的研究狀況 [J]. 普外臨床，1994; 9(4)∶193.
  2. 2. 曾勇，肖路加，張尚福. 兔膽紅素鈣結石成石過程中膽囊免疫球蛋白分泌細胞含量的變化 [J]. 普外基礎與臨床雜志，1997; 4(2)∶75.
  3. 3. Meeda T, Bismuth H, Sherlock DJ, et al. A simple and rapid method for the preparation of plasma membranes [J]. Biochim Biophys Acta, 1983; 731(5)∶115.
  4. 4. Jame L, Merion RM, Burtch GD, et al. Properties of Na+K+activated ATPase in rat liver plasma membranes enriched with bile canaliculi [J]. Biochim Biophys Acta, 1975; 401(3)∶59.
  5. 5. Schanne FA, Muller C, Endlich K, et al. Liver plasma membrane calcium transport [J]. J Biol Chem, 1986; 261(21)∶9886.
  6. 6. Michael H, Garrel T, Decurtins M, et al. Mechanisms and regulations of bile secretion [J]. Hepatology, 1991; 14(3)∶551.
  7. 7. Frimmer M, Grant B, Zobeck TS, et al. The transport of bile acids in liver cells [J]. Biochim Biophys Acta, 1988; 947(8)∶75.
  8. 8. Chung TY. Calcium and ischemic injury [J]. New Engl J Med, 1986;314(26)∶1670.
  9. 9. Victor A, Inan M, Sayek I, et al. Conditions that results in the mobilization and influx of Ca2+ into rat hepatocytes induce the rapid loss of HMGCoA reductase activity that is not reversed by phosphatase treatment [J]. Biochem J, 1990; 269(9)∶373.