目的 探索呼吸道合胞病毒( RSV) 感染的氣道上皮細(xì)胞對(duì)大鼠髓系樹突狀細(xì)胞( mDCs) 激活和功能的影響, 為研究RSV 誘發(fā)哮喘的機(jī)制提供依據(jù)。方法 大鼠氣道上皮細(xì)胞株進(jìn)
行培養(yǎng)。在對(duì)RSV 進(jìn)行擴(kuò)增和滴度測(cè)定后, 以不同作用時(shí)間、不同滴度的RSV 感染大鼠氣道上皮細(xì)胞。同時(shí)分離、培養(yǎng)擴(kuò)增大鼠mDCs, 采用transwell 細(xì)胞共培養(yǎng)系統(tǒng)將大鼠氣道上皮細(xì)胞和大鼠mDCs共培養(yǎng)。其mDCs 通過(guò)RT-PCR 和流式細(xì)胞術(shù)檢測(cè)成熟標(biāo)志物和Th2 細(xì)胞因子的表達(dá)、同種異體混合淋巴細(xì)胞反應(yīng)( MLR) 。結(jié)果 大鼠mDCs 與RSV 感染的大鼠氣道上皮細(xì)胞共培養(yǎng)后出現(xiàn)功能性成熟, 包括大鼠mDCs 表面共刺激分子MHCⅡ 和CD86 的表達(dá)增多, OX40L 和TARC 的mRNAs 表達(dá)增高, 大鼠mDCs 刺激T 細(xì)胞增殖的能力增強(qiáng)。結(jié)論 RSV 感染大鼠氣道上皮細(xì)胞能夠促使大鼠mDCs 進(jìn)一步成熟并且可能具有支持Th2 細(xì)胞分化和局部聚集的能力, 可能是RSV 誘發(fā)哮喘的一種機(jī)制。
引用本文： 喬建甌,金先橋. 呼吸道合胞病毒感染的大鼠氣道上皮細(xì)胞對(duì)樹突狀細(xì)胞的影響. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2010, 9(6): 602-605. doi: 復(fù)制
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| 3. | Zhang Y, Luxon BA, Casola A, et al. Expression of respiratory syncytial virus-induced chemokine gene networks in lower airway epithelial cells revealed by cDNA microarrays. J Virol,2001, 75:9044-9058. |
| 4. | 毛光宇, 楊炯, 陳宏斌, 等. 過(guò)敏性哮喘患者樹突狀細(xì)胞對(duì)原始T細(xì)胞活化的影響. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2005,4: 115-118. |
| 5. | Mohapatra SS, Boyapalle S. Epidemiologic, experimental, and clinical links between respiratory syncytial virus infection and asthma. Clin Microbiol Rev, 2008, 21: 495-504. |
| 6. | Lambrecht BN, De Veerman M, Coyle AJ, et al. Myeloid dendritic cells induce Th2 responsesto inhaled antigen, leading to eosinophilic airway inflammation. J Clin Invest, 2000 , 106: 551-559. |
| 7. | McAdam AJ, Schweitzer AN, Sharpe AH. The role of B7 costimulation in activation and differentiation of CD41 and CD81 T cells. Immunol Rev, 1998 , 165: 231-247 . |
| 8. | Keane-Myers AM, Gause WC, Finkelman FD, et al. Development of murine allergic asthma is dependent upon B7-2 costimulation. J Immunol, 1998, 160: 1036-1043 . |
| 9. | Lordan JL, Davies DE, Wilson SJ, et al. The role of CD28-B7 costimulation in allergen-induced cytokine release by bronchial mucosa from patients with moderately severe asthma. J Allergy Clin Immunol, 2001, 108: 976-981 . |
| 10. | Tsuyuki S, Tsuyuki J, Einsle K, et al. Costimulation through B7-2( CD86) is required for the induction of a lung mucosal T helper cell 2 ( TH2) immune response and altered airway responsiveness. J Exp Med, 1997, 185 : 1671 -1679 . |
| 11. | Imai T, Yoshida T, Baba M, et al. Molecular cloning of a novel T celldirected CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector. J Biol Chem,1996,271:21514-21521. |
| 12. | Bonecchi R, Bianchi G, Bordignon PP, et al. Differential express, on of chemokine receptors and chamotactic responsiveness of type1 Thelper cells( Thls) and Th2s. J Exp Med, 1998 , 187: 129-134 . |
| 13. | Leung TF, Wong CK. Chan IH, et al. Plasma cocentration of thymus and activatlon-regulated chemokine is elevated in childhood asthma.Jallergy Clin lmmunol, 2002, 110: 404 -409. |
- 1. 李明才, 何韶衡. 病毒感染在誘發(fā)和加重哮喘中的作用. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2003, 2: 189-192.
- 2. Becker S, Reed W, Henderson FW, et al. RSV infection of human airway epithelial cells causes production of the beta-chemokine RANTES. Am J Physiol, 1997 , 272: L512-L520 .
- 3. Zhang Y, Luxon BA, Casola A, et al. Expression of respiratory syncytial virus-induced chemokine gene networks in lower airway epithelial cells revealed by cDNA microarrays. J Virol,2001, 75:9044-9058.
- 4. 毛光宇, 楊炯, 陳宏斌, 等. 過(guò)敏性哮喘患者樹突狀細(xì)胞對(duì)原始T細(xì)胞活化的影響. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2005,4: 115-118.
- 5. Mohapatra SS, Boyapalle S. Epidemiologic, experimental, and clinical links between respiratory syncytial virus infection and asthma. Clin Microbiol Rev, 2008, 21: 495-504.
- 6. Lambrecht BN, De Veerman M, Coyle AJ, et al. Myeloid dendritic cells induce Th2 responsesto inhaled antigen, leading to eosinophilic airway inflammation. J Clin Invest, 2000 , 106: 551-559.
- 7. McAdam AJ, Schweitzer AN, Sharpe AH. The role of B7 costimulation in activation and differentiation of CD41 and CD81 T cells. Immunol Rev, 1998 , 165: 231-247 .
- 8. Keane-Myers AM, Gause WC, Finkelman FD, et al. Development of murine allergic asthma is dependent upon B7-2 costimulation. J Immunol, 1998, 160: 1036-1043 .
- 9. Lordan JL, Davies DE, Wilson SJ, et al. The role of CD28-B7 costimulation in allergen-induced cytokine release by bronchial mucosa from patients with moderately severe asthma. J Allergy Clin Immunol, 2001, 108: 976-981 .
- 10. Tsuyuki S, Tsuyuki J, Einsle K, et al. Costimulation through B7-2( CD86) is required for the induction of a lung mucosal T helper cell 2 ( TH2) immune response and altered airway responsiveness. J Exp Med, 1997, 185 : 1671 -1679 .
- 11. Imai T, Yoshida T, Baba M, et al. Molecular cloning of a novel T celldirected CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector. J Biol Chem,1996,271:21514-21521.
- 12. Bonecchi R, Bianchi G, Bordignon PP, et al. Differential express, on of chemokine receptors and chamotactic responsiveness of type1 Thelper cells( Thls) and Th2s. J Exp Med, 1998 , 187: 129-134 .
- 13. Leung TF, Wong CK. Chan IH, et al. Plasma cocentration of thymus and activatlon-regulated chemokine is elevated in childhood asthma.Jallergy Clin lmmunol, 2002, 110: 404 -409.