【摘要】 目的 建立兔耳中期瘢痕動(dòng)物模型，尋找兔耳瘢痕形成的最佳位點(diǎn)。 方法 選用日本大耳白兔20只，在兔耳腹側(cè)選定6個(gè)位點(diǎn)，作直徑1 cm直達(dá)軟骨表面的皮膚全層及軟組織缺損240個(gè)。創(chuàng)面暴露，于傷后7 d去除軟骨上面的肉芽及血漿痂殼一次。術(shù)后連續(xù)3個(gè)月觀察創(chuàng)面自然愈合及瘢痕增生情況;用HE及苦味酸-天狼星紅染色觀察瘢痕形成及膠原分布情況;用計(jì)算機(jī)圖像分析系統(tǒng)測(cè)定膠原含量。 結(jié)果 兔耳腹側(cè)可制作類似人的增生性瘢痕模型，瘢痕的發(fā)生率42.5%~56.7%，瘢痕增生的高峰在造創(chuàng)后30~50 d。不同位點(diǎn)瘢痕增生程度不同，膠原含量也不同。 結(jié)論 兔耳腹側(cè)可建立中期瘢痕動(dòng)物模型，兔耳腹側(cè)的中分和耳尖外側(cè)部分是制作兔耳增生性瘢痕的理想位點(diǎn)。
【Abstract】 Objective To establish an animal model of intermediate stage hypertrophic scar on the rabbit ears and to find out the best sites of scar formation. Methods A total of 240 full-thickness skin and tissue defect directing access to the cartilage surface was created on the ventral side in 20 Japan white rabbits and each ear contain 6 defect sites.The wound was treated by exposure method.On the 7th day after operation, the granulation tissue and plasma shell were removed on the cartilage.Wound healing and scar proliferation under natural condition were observed continuously for 3 months.The scar formation and collagen distribution were observed by HE and Sirius red staining, and the collagen content was analyzed by using computer image analysis system. Results The ventral wound of rabbit’s ears produced hypertrophic scar similar to human hypertrophic scar, the incidence of scar was between 42.5% to 56.7%.The peak of scar proliferation was in 30 days to 50 days after operation.The degree of scar proliferation and collagen content varied at different sites. Conclusion The ventral wound of rabbit’s ears can produce intermediate stage hypertrophic scar model, the middle sites and the lateral ear tip are ideal site for madding animal model of hypertrophic scar on the rabbit ears.
引用本文： 于蓉,宋燁,陳俊杰,劉曉雪,岑瑛. 一種兔耳中期增生性瘢痕動(dòng)物模型的建立. 華西醫(yī)學(xué), 2010, 25(7): 1209-1212. doi: 復(fù)制
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- 1. Morris DE, Wu L, Zhao LL, et al. Acute and chronic animal models for excessive dermal scarring: quantitative studies[J]. Plast Reconstr Surg, 1997, 100(3):674-681.
- 2. 李薈元, 劉建波, 蘭海. 建立增生性瘢痕動(dòng)物模型[J]. 第四軍醫(yī)大學(xué)學(xué)報(bào), 1998, 19(6): 655-657.
- 3. 向軍,王志勇,賈生賢,等. 一種增生性瘢痕動(dòng)物模型的建立[J]. 中華燒傷雜志, 2004, 20(5): 281-283.
- 4. Aksoy HM, Vargel I, Canter HI, et al. A new experiment hypertrophic scar model in guinea pigs[J]. Ansth Plast Surg, 2002, 26: 388-396.
- 5. 楊東運(yùn), 李世榮, 增生性瘢痕和瘢痕疙瘩動(dòng)物模型的研究現(xiàn)狀[J]. 中華整形外科雜志, 2003, 23(4): 245-251.
- 6. 李薈元. 創(chuàng)傷研究動(dòng)物模型——兔耳瘢痕模型的建立與應(yīng)用[M]. 西安: 第四軍醫(yī)大學(xué)出版社, 2005: 44-45.
- 7. 李希軍, 柳大烈, 王吉慧. 兔耳增生性瘢痕建立方法的探討[J]. 中國(guó)美容醫(yī)學(xué), 2006, 15(5):499-500.
- 8. 陶蕓, 趙亞南, 王繼華, 等. IGF-1R抗體對(duì)兔耳瘢痕組織的影響[J]. 中國(guó)美容醫(yī)學(xué), 2006, 15(12):1334-1336.
- 9. 趙自然, 劉鶴松, 張舵, 等. 人參皂甙Rg3對(duì)兔耳增生性瘢痕的抑制作用[J]. 吉林大學(xué)學(xué)報(bào). 醫(yī)學(xué)版, 2008, 34(4): 628-632.