• 1.上海交通大學(xué)附屬第一人民醫(yī)院松江分院消化科(上海201600);;
  • 2.武漢大學(xué)人民醫(yī)院消化科(武漢430060);

目的 　研究HERG在CD1小鼠膽囊組織及膽囊Cajal間質(zhì)細胞（interstitial cells of Cajal,ICC）中的表達及其意義。
方法 　采用免疫組化方法及Western blot法檢測CD1小鼠膽囊組織中HERG蛋白的表達； RT-PCR法檢測膽囊組織中HERG mRNA的表達； 雙重免疫熒光標記法檢測急性分離膽囊ICC中HERG蛋白的表達。
結(jié)果 　免疫組化結(jié)果顯示，HERG在CD1小鼠膽囊組織的細胞漿中呈黃色或棕黃色陽性反應(yīng)，主要表達于膽囊黏膜層； RT-PCR法檢測結(jié)果顯示，CD1小鼠膽囊組織中HERG mRNA陽性表達； Western blot檢測結(jié)果顯示，CD1小鼠膽囊組織中HERG蛋白陽性表達； 雙重免疫熒光標記法檢測結(jié)果顯示，CD1小鼠膽囊ICC 中 HERG蛋白陽性表達。
結(jié)論 　CD1小鼠膽囊組織以及膽囊ICC中均存在HERG的表達，HERG可能與ICC起搏功能有關(guān)。

引用本文： 孫曉敏,余保平,高禮層,徐龍,董衛(wèi)國,羅和生,徐萍. HERG在小鼠膽囊中的表達及其意義. 中國普外基礎(chǔ)與臨床雜志, 2009, 16(9): 740-744. doi: 復(fù)制

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1. Parr E, Pozo MJ, Horowitz B, et al. ERG K+ channels modulate the electrical and contractile activities of gallbladder smooth muscle [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2003; 284(3): G392-G398.
2. Pillozzi S, Brizzi MF, Balzi M, et al. HERG potassium cha-nnels are constitutively expressed in primary human acute myeloid leukemias and regulate cell proliferation of normal and leukemic hemopoietic progenitors [Ｊ]. Leukemia, 2002; 16(9): 1791-1798.
3. Lastraioli E, Guasti L, Crociani O, et al. herg1 gene and HERG1 protein are overexpressed in colorectal cancers and regulate cell invasion of tumor cells [Ｊ]. Cancer Res, 2004; 64(2): 606-611.
4. Farrelly AM, Ro S, Callaghan BP, et al. Expression and function of KCNH2 (HERG) in the human jejunum [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2003; 284(6): G883-G895.
5. Imai T, Okamoto T, Yamamoto Y, et al. Effects of different types of K+ channel modulators on the spontaneous myogenic contraction of guinea-pig urinary bladder smooth muscle [Ｊ]. Acta Physiol Scand, 2001; 173(3): 323-333.
6. Ohya S, Asakura K, Muraki K, et al. Molecular and functional characterization of ERG, KCNQ, and KCNE subtypes in rat stomach smooth muscle [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2002; 282(2): G277-G287.
7. Zhu Y, Golden CM, Ye J, et al. ERG K+ currents regulate pacemaker activity in ICC [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2003; 285(6): G1249-G1258.
8. Zhu Y, Ye J, Huizinga JD. Clotrimazole-sensitive K+ currents regulate pacemaker activity in interstitial cells of Cajal [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2007; 292(6): G1715-G1725.
9. 孫曉敏, 余保平, 高禮層, 等. 小鼠膽囊Cajal間質(zhì)細胞的初步研究 [Ｊ]. 中華消化雜志, 2007; 27(4): 225-227.
10. Schwarz JR, Bauer CK. Functions of erg K+ channels in excitable cells [Ｊ]. J Cell Mol Med, 2004; 8(1): 22-30.
11. Hua F, Johns DC, Gilmour RF Jr. Suppression of electrical alternans by overexpression of HERG in canine ventricular myocytes [Ｊ]. Am J Physiol Heart Circ Physiol, 2004; 286(6): H2342-H2351.
12. Volberg WA, Koci BJ, Su W, et al. Blockade of human cardiac potassium channel human ether-a-go-go-related gene (HERG) by macrolide antibiotics [Ｊ]. J Pharmacol Exp Ther, 2002; 302(1): 320-327.
13. Akbarali HI, Thatte H, He XD, et al. Role of HERG-like K+ currents in opossum esophageal circular smooth muscle [Ｊ]. Am J Physiol, 1999; 277(6 Pt 1): C1284-C1290.
14. Everson GT. Gallbladder function in gallstone disease [Ｊ]. Gastroenterol Clin North Am, 1991; 20(1): 85-110.
15. 別平, 范毓東. 膽囊結(jié)石發(fā)病機理的研究進展 [Ｊ]. 中國普外基礎(chǔ)與臨床雜志, 2003; 10(5): 439-441.
16. Konturek JW, Scott GW, Kingma YJ. Electrical activity of canine gallbladder [Ｊ]. Physiol Pharmacol, 1991; 42(1): 85-91.
17. Ward SM, Ordg T, Bayguinov JR, et al. Development of interstitial cells of Cajal and pacemaking in mice lacking enteric nerves [Ｊ]. Gastroenterology, 1999; 117(3): 584-594.
18. Wu JJ, Rothman TP, Gershon MD. Development of the interstitial cell of Cajal: origin, kit dependence and neuronal and nonneuronal sources of kit ligand [Ｊ]. J Neurosci Res, 2000; 59(3): 384-401.
19. Vanderwinden JM, Rumessen JJ, Bernex F， et al. Distribution and ultrastructure of interstitial cells of Cajal in the mouse colon, using antibodies to Kit and Kit (W-lacZ) mice [Ｊ]. Cell Tissue Res, 2000; 302: 155-170.
20. Ward SM, Sanders KM. Interstitial cells of Cajal: primary targets of enteric motor innervation [Ｊ]. Anat Rec, 2001; 262(1): 125-135.
21. Ward SM, Sanders KM. Physiology and pathophysiology of the interstitial cell of Cajal: from bench to bedside. Ⅰ. Functional development and plasticity of interstitial cells of Cajal networks [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2001; 281(3): G602-G611.
  1. 1. Parr E, Pozo MJ, Horowitz B, et al. ERG K+ channels modulate the electrical and contractile activities of gallbladder smooth muscle [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2003; 284(3): G392-G398.
  2. 2. Pillozzi S, Brizzi MF, Balzi M, et al. HERG potassium cha-nnels are constitutively expressed in primary human acute myeloid leukemias and regulate cell proliferation of normal and leukemic hemopoietic progenitors [Ｊ]. Leukemia, 2002; 16(9): 1791-1798.
  3. 3. Lastraioli E, Guasti L, Crociani O, et al. herg1 gene and HERG1 protein are overexpressed in colorectal cancers and regulate cell invasion of tumor cells [Ｊ]. Cancer Res, 2004; 64(2): 606-611.
  4. 4. Farrelly AM, Ro S, Callaghan BP, et al. Expression and function of KCNH2 (HERG) in the human jejunum [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2003; 284(6): G883-G895.
  5. 5. Imai T, Okamoto T, Yamamoto Y, et al. Effects of different types of K+ channel modulators on the spontaneous myogenic contraction of guinea-pig urinary bladder smooth muscle [Ｊ]. Acta Physiol Scand, 2001; 173(3): 323-333.
  6. 6. Ohya S, Asakura K, Muraki K, et al. Molecular and functional characterization of ERG, KCNQ, and KCNE subtypes in rat stomach smooth muscle [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2002; 282(2): G277-G287.
  7. 7. Zhu Y, Golden CM, Ye J, et al. ERG K+ currents regulate pacemaker activity in ICC [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2003; 285(6): G1249-G1258.
  8. 8. Zhu Y, Ye J, Huizinga JD. Clotrimazole-sensitive K+ currents regulate pacemaker activity in interstitial cells of Cajal [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2007; 292(6): G1715-G1725.
  9. 9. 孫曉敏, 余保平, 高禮層, 等. 小鼠膽囊Cajal間質(zhì)細胞的初步研究 [Ｊ]. 中華消化雜志, 2007; 27(4): 225-227.
  10. 10. Schwarz JR, Bauer CK. Functions of erg K+ channels in excitable cells [Ｊ]. J Cell Mol Med, 2004; 8(1): 22-30.
  11. 11. Hua F, Johns DC, Gilmour RF Jr. Suppression of electrical alternans by overexpression of HERG in canine ventricular myocytes [Ｊ]. Am J Physiol Heart Circ Physiol, 2004; 286(6): H2342-H2351.
  12. 12. Volberg WA, Koci BJ, Su W, et al. Blockade of human cardiac potassium channel human ether-a-go-go-related gene (HERG) by macrolide antibiotics [Ｊ]. J Pharmacol Exp Ther, 2002; 302(1): 320-327.
  13. 13. Akbarali HI, Thatte H, He XD, et al. Role of HERG-like K+ currents in opossum esophageal circular smooth muscle [Ｊ]. Am J Physiol, 1999; 277(6 Pt 1): C1284-C1290.
  14. 14. Everson GT. Gallbladder function in gallstone disease [Ｊ]. Gastroenterol Clin North Am, 1991; 20(1): 85-110.
  15. 15. 別平, 范毓東. 膽囊結(jié)石發(fā)病機理的研究進展 [Ｊ]. 中國普外基礎(chǔ)與臨床雜志, 2003; 10(5): 439-441.
  16. 16. Konturek JW, Scott GW, Kingma YJ. Electrical activity of canine gallbladder [Ｊ]. Physiol Pharmacol, 1991; 42(1): 85-91.
  17. 17. Ward SM, Ordg T, Bayguinov JR, et al. Development of interstitial cells of Cajal and pacemaking in mice lacking enteric nerves [Ｊ]. Gastroenterology, 1999; 117(3): 584-594.
  18. 18. Wu JJ, Rothman TP, Gershon MD. Development of the interstitial cell of Cajal: origin, kit dependence and neuronal and nonneuronal sources of kit ligand [Ｊ]. J Neurosci Res, 2000; 59(3): 384-401.
  19. 19. Vanderwinden JM, Rumessen JJ, Bernex F， et al. Distribution and ultrastructure of interstitial cells of Cajal in the mouse colon, using antibodies to Kit and Kit (W-lacZ) mice [Ｊ]. Cell Tissue Res, 2000; 302: 155-170.
  20. 20. Ward SM, Sanders KM. Interstitial cells of Cajal: primary targets of enteric motor innervation [Ｊ]. Anat Rec, 2001; 262(1): 125-135.
  21. 21. Ward SM, Sanders KM. Physiology and pathophysiology of the interstitial cell of Cajal: from bench to bedside. Ⅰ. Functional development and plasticity of interstitial cells of Cajal networks [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2001; 281(3): G602-G611.