目的 探討青蒿素(artemisinin)對(duì)急性胰腺炎胰腺腺泡細(xì)胞凋亡的影響,并了解是否減輕急性胰腺炎嚴(yán)重程度。
方法 腹腔內(nèi)注射雨蛙素(cerulein)誘導(dǎo)大鼠胰腺炎模型建立,設(shè)正常對(duì)照組、胰腺炎組及青蒿素組,通過原位末端標(biāo)記(TUNEL)法檢測(cè)各組胰腺組織中腺泡細(xì)胞的凋亡指數(shù),并用分光光度法檢測(cè)髓過氧化物酶(MPO)活性。采用兩步酶消化法分離正常大鼠胰腺腺泡細(xì)胞,離體分組培養(yǎng),采用丫碇橙(AO)染色及caspase3活性的檢測(cè),評(píng)估青蒿素對(duì)胰腺腺泡細(xì)胞凋亡的影響,并檢測(cè)各組培養(yǎng)液中的乳酸脫氫酶(LDH)及淀粉酶(AMS)活性,評(píng)估胰腺腺泡細(xì)胞酶的釋放。
結(jié)果 在體實(shí)驗(yàn)中,青蒿素組與胰腺炎組比較,胰腺腺泡細(xì)胞凋亡指數(shù)明顯增高(P<0.05),MPO活性明顯減低(P<0.05)。體外實(shí)驗(yàn)中,青蒿素組胰腺腺泡細(xì)胞凋亡指數(shù)及caspase3活性較胰腺炎組明顯增高(P<0.05),培養(yǎng)液中的LDH和AMS活性也明顯降低(P<0.05)。
結(jié)論 青蒿素可促進(jìn)大鼠急性胰腺炎胰腺腺泡細(xì)胞凋亡,減少胰酶釋放及中性粒細(xì)胞激活,可減輕急性胰腺炎嚴(yán)重程度。
引用本文: 張偉輝,鄭彪,薛東波,潘尚哈,史興曄,孫備. 青蒿素誘導(dǎo)急性胰腺炎胰腺腺泡細(xì)胞凋亡的實(shí)驗(yàn)研究. 中國普外基礎(chǔ)與臨床雜志, 2007, 14(4): 388-391. doi: 復(fù)制
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- 1. He ZJ, Podkletnova I, Alho H, et al. Apoptosis in acute pancreatitis[J]. Ann Chir Gynaecol, 2000; 89(1)∶65.
- 2. 李濤, 羅軍, 溫浩. 細(xì)胞凋亡在大鼠胰腺移植急性排斥反應(yīng)中的作用 [J]. 中國普外基礎(chǔ)與臨床雜志, 2007; 14(1)∶59.
- 3. Singh NP, Lai HC. Artemisinin induces apoptosis in human cancer cells[J]. Anticancer Res, 2004; 24(4)∶2277.
- 4. 李全生, 陳曉理, 周總光, 等. 雨蛙素腹腔注射法建立小鼠急性壞死性胰腺炎模型的實(shí)驗(yàn)研究 [J]. 中國普外基礎(chǔ)與臨床雜志, 2004; 11(4)∶335.
- 5. Moinfar F, Mannion C, Man YG, et al. Mammary “comedo”DCIS: apoptosis, oncosis, and necrosis: an electron microscopic examination of 8 cases[J]. Ultrastruct Pathol, 2000; 24(3)∶135.
- 6. Kaiser AM, Saluja AK, Sengupta A, et al. Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis[J]. Am J Physiol, 1995; 269(5 Pt 1) ∶C1295.
- 7. 常華, 嚴(yán)際慎, 王平瑜, 等. 缺血再灌注損傷對(duì)大鼠急性胰腺炎胰腺細(xì)胞凋亡的影響 [J]. 中國普外基礎(chǔ)與臨床雜志, 2005; 12(2)∶150.
- 8. 陳曉理, 黃興蘭, 吳浩. 急性胰腺炎血中抗炎癥細(xì)胞因子的意義[J]. 中國普外基礎(chǔ)與臨床雜志, 2000; 7(6)∶354.
- 9. 楊智勇, 王春友, 陶京, 等. 早期血液濾過對(duì)重癥急性胰腺炎家豬TNFα及IL1β水平的影響[J]. 中國普外基礎(chǔ)與臨床雜志, 2004; 11(5)∶433.
- 10. 高軍, 張淑文, 李非. 胰蛋白酶原激活肽在大鼠實(shí)驗(yàn)性急性胰腺炎中的產(chǎn)生及意義[J]. 中國普外基礎(chǔ)與臨床雜志, 2001; 8(5)∶301.
- 11. Rinderknecht H. Fatal pancreatitis, a consequence of excessive leukocyte stimulation? [J]. Int J Pancreatol, 1988; 3(2-3)∶105.
- 12. Chiu DF, Chen JC, Chen HM, et al. Results of treating severe acute pancreatitis with gabexate is associated with neutrophil apoptosis activity[J]. Hepatogastroenterology, 2003; 50(50)∶553.
- 13. Yamaguchi Y, Okabe K, Liang J, et al. The novel carboxamide derivative IS741 reduces neutrophil chemoattractant production by bronchoalveolar macrophages in rats with ceruleininduced pancreatitis complicated by sepsis[J]. Digestion, 1999; 60 Suppl 1∶52.
- 14. Hahm KB, Kim JH, You BM, et al. Induction of apoptosis with an extract of Artemisia asiatica attenuates the severity of ceruleininduced pancreatitis in rats[J]. Pancreas, 1998; 17(2)∶153.
- 15. Gallagher SF, Yang J , Bak sh K, et al. Acute pancreatitis induces FasL gene expression and apoptosis in the liver[J]. J Surg Res, 2004; 122(2)∶201.